The alcoholic extract of mulberry bark, from which the precipitate has been removed, was concentrated under vacuum and put to water distillation and obtained volatile oil (ca 0.07% of the material).
The non-volatile portion was dissolved in ether and shaken with 5% Na2CO3 and then with 2% KOH solutions. KOH solution contained a substance believed to be resinotannol. From the portion existing between ether and alkaline layers, sitosterol-d-glucoside was obtained. Ether soluble portion was saponified with alcoholic potash and α-amyrin, sitosterol and pale yellow powder (probably resen of Tschirch's) were obtained from the neutral portion. Acid portion yielded palmitic and stearic acids.