ロートエキス倍散のアルカロイド定量法に就て

抄録

Various kinds of formalizing agent is used in the preparation of the powdered Scopolia extract. These agents have great effects on the result of quantitative determination so that the method given in the Pharmacopoeae cannot be applied directly. Following method is proposed by the author for the quantitative determination of alkaloids in powdered Scopolia extract. 12g of the sample is taken to which are added 100cc ether and 10cc 15% aqueous ammonia and shaken well. 1-5cc Water is added to this and shaken for 1hr., filtered through cotton wool, 1g talc added to the filtrate, shaken, 5cc water added, shaken, 1.5g finely powered tragacanth added and shaken well. A definite amount of this ether solution is taken, filtered through a dry filter paper, evaporated over a water bath of about 60° and the residue dissolved in 1cc alcohol. To this are added 5cc n/10 HCl and about 1cc CCl₄, shaken slightly to allow colored portion to transit to CCl₄ layer and 5cc water added. This finally is titrated with n/10 KOH solution with 2 drops of methyl red as an indicator.