貝類エキス中のN-メチルピリジニウムハイドロオキサイドの檢出法

抄録

The existing method of detecting N-methylpyridinium hydroxide in animal extract involves the use of its aurate. A new method was devised whereby the base was adsorbed on cation exchange resin, Amberlite IRC-50 and a phenoxyacetic acid-formal-dehyde resin, KH-4B, and desorbed by hydrochloric acid. The acid residue is extracted with ohloroform and this is submitted to paper partition chromato graphy or paper micro-electrophoreris, and finally proved by the Dragendorff reagent. This allows detection of a minute amount of N-methylpyridinium hydroxide. Using the paper chromatographic method, 1.2mg. of this substance was detected per 1kg. of Corbbicula sandai Reinhardt.