1952 年 72 巻 11 号 p. 1393-1396
Morphine was nitrosated to 2-nitrosomorphine, and the morphine-2, 3-quinone-2-monopotassium oxime formed upon the addition of alakli to it was determined by polarograph under following conditions. By this means, it was found that the relationship id=CK is established within a range of 2×10-3 to 1×10-4 mole concentration. E1/2=1.13 volt vs (S.C.E.), kp=(I-1=C⋅kD-1⋅id-1)=1.113. The procedure follows: An aqueous solution of morphine was prepared in a range of 2×10-3 to 1×10-4 mole concentration. To 1cc. of this solution, 1.5cc. of N hydrochloric acid, and then 1.5cc. of 1 mole potassium nitrite were added, and allowed to stand at a room temperature for 1 minute to effect nitrosation. To this was added 1.5cc. of 20% potassium hydroxide solution, and its id, E1/2, and kp were determined by automatic recording between -0.4 to -1.3 V, at 25°±1°.