The Constituents of the Ovaries of Globefish. V
IPurification of Globefish Poison by Chromatography
1952 年 72 巻 2 号 p. 187-190
詳細
1952 年 72 巻 2 号 p. 187-190
Crude poisons from globefish ovaries was purified by starch column partition chromatography and by adsorption chromatography using active charcoal. In both cases, amino acids and reducing sugars mixed with crude poison were found to flow at a faster rate than the poison by development. The separation of the poison and amino acids was especially complete in the case of active charcoal. Globefish poison thus obtained showed a potency of 0.6 MLD/g. mouse and when dried, became colorless, and brittle, resin-like solid containing some crystals. This is still not completely pure. The amino acids mixed in the poison were identified by paper chromatography by which it was seen that these amino acids are found in the hydrolysate of the ovary tissues of globefish.
