1952 年 72 巻 4 号 p. 585-588
1) Transmission maximum of four kinds of filters was determined by the Beckman spectrophotometer for use in the colorimetric determination of the reddish purple color that appears upon the addition of 1% NaOH to the acetone solution of santonin-2, 4-dinitrophenylhydrazone. They have a transmission maximum at 500 mμ, but some of the filter were transparent above 650 mμ. The inclination and linearity of concentration-absorption curves decrease when, these filters are used. Two kinds of filters were prepared for the present experiments which gave more pronounced inclination and linearity to the curves but which were opaque above 650 mμ.
2) Filters must be kept in a dark place to avoid discoloration, and their properties must be checked from time to time.
3) By selenium-cell filter photometer, light absorptions (ε) were measured with the prepared filter in different concentrations (γ). The experimental equation was calculated from these data as follows: γ=1445ε+14.12
4) The standard deviation, δ, is 0.021, and the concentration limit, which corresponds to 2δ limit, is ±42.4γ.