ビタミンCの螢光反応 (第5報)

反応機序に就いて

抄録

In order to understand the reaction mechanism whereby a solution of dehydroascorbic acid shows fluorescence upon the addition of o-phenylenediamine solution, 2-(l-glycerodihydroxyethyl)-3-ketofuro [2, 3-b] quinoxaline (I), 7-(l-glycerodihydroxyethyl)-13, 14-furo [2, 3-b; 3, 4-b′] diquinoxaline (II) and 3-(l-glycerodihydroxyethyl)-1-ketofuro [3, 4-b]-quinoxaline (III) were prepared and the nature of the fluorescence shown by the aqueous solutions of these compounds, Rf values of the fluorescent substance, ultraviolet absorption spectra, and the properties of respective phenylhydrazones were examined. It was found from these experimental results that the only one that gave completely identical properties as the reaction mixture was that from (I).