1953 年 73 巻 4 号 p. 329-331
Protease was extracted with water from hog pancreas and activated by standing the solution at 0°C for 72 hours. This extract was used for the enzymatic hydrolysis of casein, and the decomposition rate (α-amino-N/total-N, %) was determined with samples taken at definite intervals. At the same time, free and total methionine were determined by T. F. Lavine's method, free tryptophane by Shimada's method, and total tryptophane with p-dimethylaminobenzaldehyde, to examine the rate of methionine and tryptophane liberation and decomposition during the course of enzymatic hydrolysis. The results are shown in Table I, and the relationship between the rates of methionine and tryptophane liberation is shown in Fig. 1.