2-Phenylcinchonic Acid Hydrazide及びp-Aminosalicylic Acid Hydrazideの研究

抄録

2-Phenylcinchonic acid hydrazide (I), m.p. 227°, and p-aminosalicylic acid hydrazide (II), m.p. 199-200°, were prepared by the method of Curtius and Struve, and their toxicity, LD₅₀, against mice, in vitro antibacterial action against human type Aoyama strain tubercle bacilli and non-pathogenic acid-fast bacteria No. 920 were tested in Proskauer and Beck modified medium (10% bovine serum added). (I) was able to inhibit the growth of Aoyama strain at 1000γ/cc. concentration in a 4-weeks test but possessed no antibacterial power against No. 920 strain. LD₅₀ by subcutaneous injection in mouse was found to be 12mg./10g., the toxic symptoms being sedation, difficulty of walking, drowsiness, convulsion of the limbs, difficulty of breathing, and respiratory paralysis. LD₅₀ of (II) by subcutaneous injection in mouse was 1.5mg./10g., the toxic symptoms being excitement, unrest, paralysis of hindlimbs, difficulty of breathing, spasm, and respiratory paralysis. (II) was able to inhibit growth, of Aoyama strain at 10γ/cc. concentration, and in 1000γ/cc. concentration against acid-fast bacteria No. 920 in a 4-week test.