YAKUGAKU ZASSHI
Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
Ninhydrin反応に関する研究 (第1報)
α-アミノ酸の比色定量法
山岸 正治吉田 慰子
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1953 年 73 巻 7 号 p. 675-676

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It was found that colorimetric determination of α-amino acids was possible by reacting them with ninhydrin in pyridine, in the pressence of ascorbic acid. The experimental error was less than 5%, and the minimum amount of α-amino acids possible to be determined was ca. 0.5 γ converting into amino-nitrogen. The hydrindantin produced from ninhydrin and ascorbic acid, pyridine, and an excess of ninhydrin all had an effect of preventing the decomposition of the pigment, diketohydrindylidenediketohydrindamine.

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