蛋白酵素分解物の薬学的研究 (第5報)

各種蛋白加水分解物中のヒスタミン, チラミン

抄録

Histamine and tyramine are assumed to be one of the substances responsible for sideeffects attending the intravenous administration of protein hydrolysate preparations. Detection of histamine and tyramine and approximate determination of their contens in various protein hydrolysates and marketed parenteral preparations were carried out by paper chromatography in reference to the method of Urbach. The results obtained are shown in Figs. 1 and 2, and in Table I. These results indicated that histamine and tyramine were present in casein hydrolysate prepared with toluene as the antiseptic and the marketed parenteral preparations in larger amounts than was expected. It was also found that there were a difference in the amount of histamine formed by the manner of hydrolysis and that in protein hydrolysate preparations obtained by alkaline hydrolysis, care must be taken to remove these harmful amines.