1953 年 73 巻 8 号 p. 841-844
Extracts of several kinds of tonic crude drugs were added to synthetic medium not containing nicotinic acid and the growth of Lactobacillus arabinosus was examined. Growth was found in all the media and the content of total nicotinic acid calculated from the degree of growth was 10-135γ/g., with an average of 38γ/g. In order to see whether this growth was actually due to nicotinic acid or not, examination was made by paper chromatography using Nuphar japonicum as the sample and a growth of microörganism was found in the portion giving the same Rf value as that of the standard nicotinic acid. By the same method, presence of vitamin B12 was proved in Ligusticum acutilobum the fact of which is significant as this crude drug is used for anemia.