コリスチンの研究 (第1報)

コリスチンのペーパークロマトグラフィー

抄録

Colistin, an antibiotic substance produced by a spore-forming bacillus discovered in Japan, was submitted to paper chromatography and the antibacterial chromatogram appearing on the paper placed on an agar plate of Escherichia coli N. I. H. J. was observed. As a result, it was seen that a large number of samples gave a clearly separated two images, and some gave a third image. The paper chromatography was carried out with Toyo Roshi No. 50, treated with 0.2M glycine buffer of pH 2.5, devloped with butanol, and three images were defined at Rf 0.53 (colistin-A), 0.38 (colistin-B), and 0.25 (colistin-C). Under the same conditions, polymixin-B gives the image at Rf 0.59. Further, separation of colistin-A and-B was effected by column chromatography by passing a mixture of colistin-A and-B through a column filled with silica gel saturated with 0.05M glycine buffer and developed with butanol.