1954 年 74 巻 12 号 p. 1341-1343
Canavanine is used in biological experiments as an antimetabolite of arginine but suitable method has not been found for separatory detection of these two substances in a sample. It has been found that good results can be obtained in this direction by coloration after separation of the two substances by paper electrophoresis in Veronal buffer (pH 8.6) or by buffered chromatography with phenol and borate buffer (pH 9.3). The colorimetric determination of canavanine in the presence of arginine was found to be impossible in a biological system because of an obstruction by metabolites but a few knowledge were gained on the determination of arginine in the presence of canavanine. For example, the determination of arginine by the Sakaguchi reaction can be freed from the influence of canavanine if hypobromite is used but coloration is interfered by the presence of a reductive substances such as glucose. Microbiological assay using Streptococcus faecalis R is not affected by the growth inhibition of canavanine nor by other arginine homologs but stimulation of growth by canavanine appears and the determination values obtained are only relative. Separation of canavanine and argininewwas effected by the use of an jon exchanger, Amberlite IRC-50 or IRA-400.