生薬中の微量成分の研究 (第5報)

ビオチンについて その1

抄録

Free type and bonded type biotin in several kinds of crude drugs, including cinnamon and liquorice, were examined by microbiological assay method. It was found that although bonded biotin was not contained in the cinnamon, other crude drugs contained both types. In order to find out whether the biotin-like factors contained in the crude drugs, as shown by the microbiological assay, were really biotin or not, paper partition chromatography was carried out. To determine the Rf value of standard biotin, it was developed with water-saturated butanol and examined by bioassay by which it was found that biotin was developed into two groups giving Rf values of 0.2-0.3 and 0.6-0.7, and both helping growth of Lactobacillus arabinosus. Biotin-like factors of liquorice, royal jelly and Apis melifica were examined under the same conditions by which growth factors were found in those giving Rf 0.2-0.3 from liquorice and royal jelly, and Rf 0.2-0.3 and 0.6-0.7 from Apis melifica.