生薬中の微量成分の研究 (第6報)

ビオチンについて その2

抄録

Paper partition chromatography of biotin was carried out with a mixture of AcOH: H₂O:BuOH (1:5:4) as the developer. Later examination by microbiological assay showed that the compound had been developed into two groups (Rf 0.6-0.7 and Rf 0.8-0.9) of substances which helped the growth of Lactobacillus arabinosus. Two-dimensional paper chromatography of the two components showed that the growth factor of larger Rf value changed to that of a smaller Rf value. It was observed that such phenomenon was affected by the heating and irradiation of ultraviolet light after spotting of biotin on the filter paper.