1954 年 74 巻 6 号 p. 690-692
In order to study the determination method for glucuronic acid, coloration of 20 kinds of aromatic amines and glucuronic acid was examined. It was thereby found that coloration with 4, 4′-diaminodiphenyl sulfone was comparatively good. Various conditions for its use in the determination were examined and the following procedure was found to be satisfactory. To 1 cc. of the sample, 5 cc. of 1% solution of 4, 4′-di-aminodiphenyl sulfone in 3.5% hydrochloric acid and 3cc. of conc. hydrochloric acid are added, and the whole volume is brought to 10cc. with water. After warming this on a boiling water bath for 30 minutes, it is cooled, allowed to stand for 35 minutes, and filtered. The absorbence at 500mμ is determined with the Beckman photoelectric spectrophotometer and the concentration of glucuronic acid is obtained from the detection curve preliminarily calculated. The accuracy is within 10%. This method is not so satisfactory for the determination of glucuronic acid but is advantageous in that it is not hinderd by the presence of other neutral sugars.