1954 年 74 巻 8 号 p. 846-849
1) A brown color appeared when the solution of hydroquinone and hydrochloric acid was added to the alcoholic solution of ascaridol, and the light absorbancy (e) of the colored solution of different concentrations (γ) was measured by the Beckman spectrophotometer (λ=400 or 425mμ). From these experimental data (n=45), the following equations were calculated by the method of least squares:
at 400mμ x=308.7y+15.7, at 425mμ x=473.5y+16.1
2) The standard deviation, σ, is ±0.0217 and the concentration limit corresponding to 2 σ limit is ±17.25mg. when measured at 400mμ.
3) The influence of a low boiling fraction of the chenopodium oil on the method was found to be negligible.
4) The data obtained by iodometry (N.F.), the Nelson's method (J.P.VI), and the present colorimetric method were compared.