抗生物質の研究 第8報

Paper ChromatographyによるTetracycline群およびVitamin B₁₂の定性的分離

抄録

A paper chromatographic technique whereby the compounds of the tetracycline series separated from each other or from vitamin B₁₂ was devised by impregnating various kinds of filter paper with Theorell buffer of varying pH and developing with the upper layer of a freshly prepared mixture of butanol, acetic acid, and water (4:1:5). In order to obtain a definite Rf values, the filter paper chosen must show no fluorescence, no absorption, and small adsorption. The Theorell buffer is adjusted to pH 3.5-6.2 and in order to impregnate a definite amount of the solution in the filter paper, the value of (a_S-a_W)/a_W was fixed at 3.0-4.0. The objective was attained by developing with the above solvent mixture by the the one-dimensional ascending method.