1955 年 75 巻 7 号 p. 799-801
When FAD was decomposed with hydrochloric acid, the product developed on a paper with butanol: acetic acid: water (4:1:5), and examined under ultraviolet light, absorption band, in regions corresponding to Rf 0.44-0.46, was detected at the borderline between adenosine and adenine, besides the bands of FAD, FMN, riboflavin, AMP, and lumiflavin. In the same chromatogram a benzidine-positive band with Rf 0.11 was detected, but it does not correspond to that of the ribose produced by the decomposition of FAD because ribose shows Rf 0.28 under the same conditions. Although many bands positive to phosphate ion reaction were detected in the chromatogram, the benzidine-positive band is also positive to the reaction. It is reasonable to suppose, therefore, the benzidine-positive band to be ribophosphate and that with Rf 0.44-0.46 for adenine. From the result, it became necessary to correct the authors' previous report on the decomposition of FAD by hydrochloric acid.
Decomposition of FAD by alkali is very complicated, and is, therefore, not suitable as a tool for the identification of FAD prepared by the authors.