無発熱性医薬品の製造に関する研究 (第5報)

Pyrogenの酸素極大波抑制に関するポーラログラフ的検討

抄録

Effect of the removal of pyrogen through quinone treatment and its reaction course were studied by polarography and this result was used in elucidating the mechanism of pyrogen removal by this means.
1) Pyrogen culture filtrate, even in a dilute solution, was found to possess extremely great action of inhibiting maximum oxygen wave and the limit of detection was 1γ/cc. as converted to the concentration of casein.
2) Pyrogen was separated into P_α and P_β (cf. next paper) by electrophoresis and maximum oxygen wave of each was measured. Both were found to show inhibitive action.
3) The solution obtained by the treatment of pyrogen culture filtrate or pyrogenic glucose parenteral solution with quinone, giving negative tetrabromophenolphthalein (TBP) reaction, is entirely devoid of suppression of maximum wave and did not indicate the presence of pyrogen by polarography. Polarographic examinations were made with sodium citrate and pyrogenic substances giving positive TBP reaction, such as egg albumin, casein, peptone, nucleic acid, brucine, and 8-hydroxyquinoline. They all gave similar results.
4) The foregoing samples were treated with quinone and the activated carbon thereby obtained was digested with redistilled water, affording solution giving positive TBP reaction and again showing suppression of maximum wave.