1955 年 75 巻 9 号 p. 1155-1156
Aromatic aldehydes and their semicarbazones and thiosemicarbazones react with peptone or tryptophan, in hydrochloride acidity, to show violet, blue, or greenish coloration. This coloration was utilized in the microdetection of such compounds. To a minute amount of the sample or its ethanolic solution, approx. 10-20mg. of peptone or 2 drops of 1w/v% of tryptophan solution and 0.5cc. of conc. hydrochloric acid are added, and the mixture is heated in a water bath for 1-2 minutes. Limit of detection using peptone: 3 γ of p-dimethylaminobenzaldehyde.