植物成分の微量化学的研究 (第22報)

瀘紙クロマトグラフィー及び瀘紙電気泳動法によるモルフィン型アルロカイドの分離並びに検出について

抄録

Separation of morphine, ethylmorphine, heroine, codeine, and hydrocodeine by buffer chromatography and paper ionophoresis was examined. Buffer chromatography at various pHs between pH 1 and 12, utilizing the method of McFarren, effected a considerable difference in the Rf values of ethylmorphine and heroine, and between codeine and hydrocodeine, which are difficult to separate on the filter paper. Comparative examination of detection of several alkaloids for minimum detectable range indicated platinum chloride-potassium iodide reagent to be the most sensitive.
Paper ionophoreses were carried out at various pHs using the Sørensen and Clark-Lubs buffer solutions and the best results were obtained at pH 9-11. Migration with the use of 0.01-1% ammonia water was found to effect marked change in the migration distance in accordance with a slight change in the concentration of ammonia water. By selecting a suitable concentration, all the alkaloids could be separated clearly from each other.