1956 年 76 巻 6 号 p. 682-686
Further purification of the gonadotropic hormone of 400-600 I. U./mg. purity was attempted by column chromatography through Hyflo Super-Cel under various conditions and a product of 2000 I. U./mg. was obtained. The product obtained by ethanol purification was found to be composed of at least two components by paper electrophoretic analysis and these two components were separated by chromatography. Both these substances were assumed to be almost homogeneous by electrophoretic analyses and both possessed gonadotropic effect.