細菌代謝産物のコリンエステラーゼ活性に及ぼす影響 (第2報)

培地に各種炭水化物, アミノ酸を添加した場合の実験

抄録

Escherichia coli was cultured at 37° for 48 hours in (1) peptone water added with 1% of one of 19 kinds of carbohydrate, (2) bouillon and (3) synthetic media each added with 1% of one of 16 kinds of amino acids. Turbidity of such culture media and the inhibition of serum cholinesterase activity by their filtrate were examined. It was thereby found that, in the case of (1), addition of salicin, dulcitol, sorbitol, inositol, and sucrose was found to effect inhibition, and in the case of (2), the inhibition was the strongest when tryptophan was added, followed by alanine, isoleucine, and phenylalanine in that order, while in (3), the inhibition was in the order of l-cystine, tryptophan, and isoleucine. Inhibition of cholinesterase activity by tryptophan was assumed to be due to tryptamine and indole formed by its decomposition by Escherichia coli but in the case of other substances, further detailed examinations are warranted.