1958 年 78 巻 11 号 p. 1211-1215
A separatory determination of pyrazinamide and its biological decomposition products excreted in urine, after concurrent administration of pyrazinamide and INAH, had been devised and this was utilized in comparative examination of biological changes of pyrazinamide after its single administration and in conjunction with INAH. The sample urine was treated with anion exchange resin (Amberlite IRA-400) to separate pyrazinoic acid and urine components, the effluent solution was oxidized with bromine to change INAH and its metabolites to isonicotinic acid, and pyrazinamide was determined by the cyanogen bromide and sodium nitroprusside methods. It was thereby found that biological change of pyrazinamide was almost the same whether administered singly or with INAH and pyrazinoic acid was excreted in far larger amounts than pyrazinamide.
Separatory determination of acetylated INAH and isonicotinic acid in urine was effected by the use of cyanogen bromide method, anion exchange resin, and acid-treated alumina, and excretion of these metabolites into urine after single administration of INAH and in conjunction with pyrazinamide was examined. As a result, acetylation rate of INAH was found to differ not greatly by its administration alone or with pyrazinamide, but excretion of isonicotinic acid was found to increase fairly greatly by the concurrent use of pyrazinamide.