毛管分析法によるタンニン生薬試験法の研究 (第2報)

フェノール化合物とメチレンブルウの反応並びにタンニン生薬及びタンニン材料についてのメチレンブルウD化法による定量値と皮粉使用の定量値との関係について

抄録

Relative reaction of phenol compounds and methylene blue was examined and it was found that phenol and compounds with a hydroxyl in the ortho position, like pyrocatechol, did not undergo D-fication reaction with methylene blue, while those with hydroxyls in mete- and para-positions, such as resorcinol and hydroquinone, those with three hydroxyls, such as pyrogallol and phloroglucinol, and compounds considered to be phenol derivative, such as catechin, rutin, woogonin, baicalin, quercitrin, were found to undergo D-fication.
Determination of tannin-bearing crude drugs and tannin materials was carried out by the methylene blue-D-fication and by the use of hide powder, and it was found that the values obtained by the two methods were in parallel and that the value from the former method multiplied by a definite coefficient agreed well with the value obtained by the latter method. It was also clarified that the difference of coefficient in various plants was inevitable from the difference in the composition of tannin in each plant and by the presence of non-tannic substance.