1958 年 78 巻 4 号 p. 346-350
In order to examine the chemical properties of callicrein, examinations were made on the method of purifying callicrein to obtain it in high purity. Callicrein is contained in hog pancreas and submaxillary gland in a large quantity. The acetone powder of hog pancreas was extracted with sodium hydrogen carbonate and the extract was purified through acetone, ethanol, activated charcoal, alumina, salting out with ammonium sulfate, and dialysis, and a sample of 200U/mg. was obtained. However, this substance was not homogeneous yet and seemed to contain two components by paper electrophoretic analysis. The callicrein obtained was inactivated by the serum of a dog. Bioassay of its potency was made by measuring depression of carotid arterial pressure in a dog. Kallikrein (Bayer) was used as the standard. Therefore 1 unit corresponds to 1 Frey unit.