1959 年 79 巻 4 号 p. 476-478
An attempt was made for fluorometric determination of reserpine which had been separated on a filter paper by electrophoresis. The fluorescence intensity of reserpine is stronger in acid solution than in neutral or alkaline solution. Various amounts of reserpine were spotted on a Whatman No. 1 filter paper and submitted to paper electrophoresis, with 5 N acetic acid as electrolyte, at 700 V for 2 hours. After migration, the paper with separated reserpine spot was heated at 105° in mixed vapor of hydrogen peroxide and acetic acid. It was thereby found that a linear relationship held between the amount of reserpine and intensity of fluorescence. The amount of reserpine present in several alkaloidal fractions of the Rauwolfia extract was also examined. In this case, the reserpine fraction must be separated from strong bases by extraction with buffered solution before electrophoresis in order to avoid error.