分配クロマトグラフィーによるアヘンアルカロイドの分離定量 (第1報)
塩酸アヘンアルカロイドの定量について
1960 年 80 巻 2 号 p. 196-200
詳細
1960 年 80 巻 2 号 p. 196-200
Determination of opium alkaloid hydrochloride specified in the Japanese Pharmacopoeia VI does not specify the method of determinating secondary alkaloids. Determination of six main alkaloids, in opium alkaloid hydrochloride, morphine, narceine, narcotine, papaverine, thebaine, and codeine, was carried out by the separation of componental alkaloids by partition chromatography and non-aqueous titration with 0.005N p-toluenesulfonic acid. The separation was effected by the addition of the sample into the alkaline column (pH 12.5) and acid column (pH 2.2), placed vertically and connected above and below, and first extracted with ether, to be separated into amphoteric and non-amphoteric bases. The columns were disconnected and each alkaloid was separatory eluted out as shown in Table I.
