リボ核酸のStreptolysin-S増産効果発現上必要なCofactorに関する研究 (第1報)
肉汁よりのCofactorの分離試験
1960 年 80 巻 7 号 p. 941-947
詳細
1960 年 80 巻 7 号 p. 941-947
Presence of a cofactor or cofactors necessary for increased production of streptolysin-S of Streptococcus by ribonucleic acid in peptone or meat infusion had already been assumed by Bernheimer and by Hosoya and Egami. In order to purify and separate this factor from meat infusion, various examinations were made and a non-dialyzable polypeptide was successfully separated which indicated a high degree of cofactor activity biologically and which was identified as chemically uniform. The route of this separation was as follows: Removal of a substance precipitating with alcohol; collection of a substance from its supernatant, precipitation with acetone, removal from this acetone precipitate a substance precipitating in ammonia alkalinity (pH 8.6), and dialysis of the centrifugation supernatant against distilled water. The dialysate was passed through a column of Amberlite IRA-411 (Cl form) and the initial effluent therefrom was dialyzed, followed by lyophilization.
