赤外部吸収スペクトルによる医薬品分析の研究 (第4報)
赤外部吸収スペクトル法による5α-Cholestan-3α-olの定量
1961 年 81 巻 1 号 p. 93-96
詳細
1961 年 81 巻 1 号 p. 93-96
Determination of 5α-cholestan-3α-ol (I) was carried out by the use of its absorption at 1002cm-1 in its infrared spectrum (Fig. 2) as the key band, in chloroform solution and as KBr pellet. From the route of its synthesis, (I) was expected to be contaminated with cholesterol (II), 5α-cholestan-3β-ol (III), and 5β-cholestan-3-one (IV), and their effect on the determination of (I) was also examined. Presence of (II) and (IV) was found not to affect the determination but (III) was found to interfere, and, therefore, determination of (I) in a mixture of (I) and (II) was attempted. As the results indicated in Tables I and II show, the standard deviation presumed according to the method of Youden was 0.55% by the solution method and 0.58% by the pellet method.
