1961 年 81 巻 12 号 p. 1703-1705
The amount of hyoscyamine (I) and scopolamine (II) contained in both Scopolia Extract and Belladonna Extract differ markedly in their ratio in these two extracts (Table I) and it is possible to differentiate these extracts by separatory determination of the two alkaloids. The minute amount of (II) contained in the Belladonna Extract can be easily detected in the Scopolia Extract by paper partition chromatography and it was confirmed that this procedure in capable of distinguishing the extract containing at least within 0.1% of the alkaloid. Norhyoscyamine (III) gives a characteristic and stable lemon yellow coloration to Ninhydrin reagent, a coloring reagent for amino acids, but other tropane alkaloids and a few betaines color reddish violet to brown with this reagent (Table II), so that its presence in the extract can be detected by paper partition chromatography. In an experiment carried out, the presence of (III) in 1g. of Scopolia Extract containing within 0.1% of alkaloids was detected but this was impossible with Belladonna Extract. Consequently, this method was found to be a good measure in distinguishing the two kinds of extract.