クロマトグラフィーによるマオウ・アルカロイドの分離定量 (第1報)

メチルエフェドリンとエフェドリンまたはプソイドエフェドリンとの分離定量

抄録

Separatory determination of Ephedra alkaloids has not been established as yet. By the application of partition column chromatography or adsorption chromatography, Separatory determination of l-methylephedrine from l-ephedrine or d-pseudoephedrine was successfully carried out. In the case of partition chromatography, Celite 545 was used as the carrier, buffer solution of pH 6.4 as the stationary phase, and chloroform as the moving phase to separate l-methylephedrine from the sample of alkaloid mixture. The use of chloroform-ethanol mixture (5:1 by volume) as the moving phase then separated l-ephedrine or d-pseudoephedrine. In adsorption chromatography, Brockmann's alumina was used as the adsorbent and the mixture sample was developed with 85% butanol-ether mixture (1:50 by volume) to separate l-methyl-ephedrine. Development with 85% ethanol-ether mixture (1:1 by volume) eluted the remaining l-ephedrine or d-pseudoephedrine. Each of the alkaloids separated by chromatography was determined by nonaqueous or neutralization titration.