YAKUGAKU ZASSHI
Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
アトロピンおよびスコポラミンの濾紙電気泳動法による分離定量
山口 一孝伊藤 巳代子
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ジャーナル フリー

1961 年 81 巻 2 号 p. 179-181

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Atropine and scopolamine were detected quantitatively by paper electrophoresis using 0.1N ammonia solution as the electrolyte. The alkaloids separated on the filter paper were detected as brown spots when exposed to iodine vapor. After elution of these spots, the solvent was evaporated, the alkaloidal residue was nitrated, dissolved in dimethylformamide, and tetraethylammonium hydroxide was added, according to the method of Freeman. The absorbancy (y) of each solution at 545mμ was measured and concentration (x in γ/cc.) of each alkaloid was calculated from the following equations:
For atropine: x=113y+0.8
For scopolamine: x=131y+1.3
Assay results from this method were compared with that of the official method, using scopolia extract and scopolia root as samples.

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© by the PHARMACEUTICAL SOCIETY OF JAPAN
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