1961 年 81 巻 2 号 p. 282-286
In accordance with the reaction mechanism for removal of pyrogen by quinone-activated carbon treatment described in the preceding report, examinations were made on the practical operational conditions for complete removal of pyrogen. Several injections of various substances containing pyrogen were submitted to the experiments under the conditions described below. In the quinone-treatment reaction, it is fundamentally essential to have all the pyrogen (P) present in the sample form the irreversible compound, PQ. The conditions for this reaction are the finely pulverized α-naphthoquinone as the quinone, in approx. 0.3g./100cc. of the injection, the solution to be maintained at neutral or slightly alkaline state, and the heating to be effected at 100° for 1-3 hours. Practical injection to be used as a sample for removal of pyrogen are glucose, pyrogenic glucose, fructose, invertose, Ringer solution, sodium alginate, dextran, and sodium citrate. Evaporated tap water was also used as a sample.
It was thereby found that this treatment was able to reduce pyrogen to the extent undetectable by the modified TBP coloration, i.e. below 0.257γ/cc., in all the samples examined in which the original content of pyrogen was 0.63-7.5γ/cc. calculated as egg albumin.