Colorimetric determination of cycloserine is made by addition of phosphotungstic-phosphomolybdic acid reagent (the so-called Folin-Ciocalteu's reagent) to the aqueous solution of cycloserine, basification of this solution with sodium carbonate, and measurement of the optical density of the resultant blue solution at 770mμ. The concentration of cycloserine is calculated from the regression equation. The value obtained by this method was found to agree well with those obtained by biological assay and by ultraviolet absorption method.