アヘンアルカロイドの分析研究 (第12報)

コデイン, モルヒネの新呈色反応とその比色定量分析への応用 その2

抄録

Examinations ware made on the analysis of morphine by ceric ammonium nitrate-2, 4-dinitrophenylhydrazine method. The limit of detection by this method is 1.5γ/0.05cc. Thebaine and papaverine interfere in this determination and both must be removed by a simple separation procedure. Narcotine did not affect the resulting data but there is a fear of its having caused secondary reaction, so that its separation was also examined. The procedure for this determination is the same as that described for codeine, with ceric ammonium nitrate as 0.4% solution and period of reaction at 20 minutes. When codeine is present, the test solution is shaken with chloroform in the presence of sodium hydroxide, and morphine is determined with the aqueous layer, codeine with the chloroform layer. When thebaine is present, morphine is determined with the aqueous layer obtained by treatment as above. When both narcotise and papaverine are present, they are separated by treatment as in the case of codeine, and morphine is then determined.