1961 年 81 巻 7 号 p. 1027-1030
Two kinds of mixed samples containing morphine, codeine, thebaine, narcotine, and papaverine were prepared according to the formula given for assay of alkaloidal content in opium and that described in Pharmacopoeia Kelvetica (1941) for analytical purpose. It was learned that determination can be made with an accuracy of within 3%. Composition of the synthetic samples were as follows: Morphine:codeine:thebaine:narcotine:papaverine=(i) 33:3.3:1:16.5:2.35 and (ii) 30:1:1:13:2. In both cases, determination is possible with the minimum amount of 1mg. of thebaine. Separatory determination of these alkaloids is carried out as follows: A sample is basified with sodium hydroxide and shaken with chloroform to transit morphine to the sodium hydroxide layer and other alkaloids to the chloroform layer (narcotine would be present in both layers and will have to be assayed with a separate sample). Sodium hydroxide layer is neutralized and morphine in it is determined. A part of the chloroform layer is shaken with 0.2% tartaric acid to transit codeine and thebaine into the aqueous layer, and thebaine is determined with a part of this aqueous solution. Another part of the aqueous solution is treated as described in Part XI of this series to separate thebaine and codeine is determined with residual solution. A separate sample is basified with ammonium hydroxide, extracted with chloroform, and papaverine and narcotine are separatory determined by the methods described in Parts VIII and X of this serles.