1961 年 81 巻 7 号 p. 994-995
The Rf values in partition-type paper chromatography contains numerous variables affected by temperature (equation (1) in the text) and its fluctuation is complicated. In order to simplify this, the value was converted to Rf value of (Vs+λs)/Vm=1/2 (Rfu value) and its relation to temperature was examined. It was found that the Rfu value is affected by the mutual solubility curve of the solvent in a two-componental solvent system and, when the solubility increases with temperature (especially when there is an upper critical solution temperature), it converges near a specific point around 0.7 with increasing temperature. When the solubility decreases with increasing temperature (especially when there is a lower critical solution temperature), the curve diverges from the specific point. It is considered that utilization of this phenomenon would make it possible to predict a suitable temperature for separation of substances.