1962 年 82 巻 11 号 p. 1493-1496
The modified Carol-Banes method for reserpine-rescinnamine assay was discussed. It was considered that such procedure would be unlikely to find favor as a routine work. The other recommended assay was proposed, in which the weakly basic alkaloidal fraction was separated by the extraction method, then hydrolysed to the acidic fraction and alkamine component. The ether-soluble acidic fraction, containing trimethoxybenzoic acid and trimethoxycinnamic acid exclusively derived from reserpine and rescinnamine, was determined spectroscopically.
The content of these alkaloids in domestic plant was found to be as follows: Average content in first-year growth, 0.109, 0.044%; second-year growth, 0.088, 0.041%; third-year growth, 0.071, 0.040%; fourth-year growth, 0.083, 0.051%. The highest content of reserpine plus rescinnamine was observed in August and the lowest in December.