医薬品の吸収と排泄に関する研究 (第5報)

クロラムフェニコールの尿中濃度測定法ならびに体内変化率の測定

抄録

In vivo change of chloramphenicol was examined by measuring urinary excretion of chloramphenicol. One cc. of urine obtained after administration of chloramphenicol was placed in a glass-stoppered test tube, 1cc. of the Clark-Lubs buffer (pH 7) and 3cc. of isoamyl acetate were added, and shaken for 10 minutes. The mixture was centrifuged, 2cc. of the supernatant was mixed with 1cc. each of 3% isonicotinic acid hydrazide solution and 6% sodium hydroxide solution, and shaken in a thermostat of 30° for 30 minutes. This supernatant was decanted and absorbance of the colored underlayer was measured (filter, S-40; light path, 5mm.). The urine obtained before administration of chloramphenicol was treated in the same manner to be used as the control and urinary concentration of chloramphenicol was obtained from the calibration curve. At the same time, total amount of nitro compound excreted was measured by the diazotization method and the amount of chloramphenicol changed in the body was obtained from the difference in the value of these amounts.