血清パロチンの研究 (第3報)
各種プロテアーゼによる血清パロチンの分解
1962 年 82 巻 3 号 p. 429-433
詳細
1962 年 82 巻 3 号 p. 429-433
It was considered that application of various proteases to serum parotin, obtained from bovine serum, might afford low-molecular substances with parotin-like activity, and trypsin, chymotrypsin, pepsin, and papain were reacted with serum parotin. Examination of the calcium-lowering and leucocyte activities of the resultant hydrolyzate showed that the calcium-lowering effect was increased than that of the control after 40 hours with trypsin or 24 hours with pepsin. Papain was found to cancel the calcium-lowering effect of serum parotin but inversely increased its action to increase the leucocytes. Chymotrypsin did not seem to change either of the effects of serum parotin. From the ultraviolet absorption and paper chromatography of the various enzymatic hydrolyzates, it was found that substances not precipitating with trichloroacetic acid increased with increasing length of incubation time.
