1963 年 83 巻 10 号 p. 1000-1004
Colorimetric determination of thebaine was examined by the use of its coloration with 3-methyl-2-benzothiazolinone hydrazone. Treatment of thebaine with dilute hydrochloric acid, reaction with 3-methyl-2-benzothiazolinone hydrazone with ammonium cerium sulfate in acidity, and basification with triethanolamine produces a reddish violet color which has an absorption maximum at 550mμ. Determination of thebaine by this method was found to be possible with good sensitivity. Presence of codeine, narcotine, and papaverine, in an amount twice that of thebaine, does not interfere in this coloration. Morphine does not interfere in this reaction as long as its amount is one-half that of thebaine but causes positive error above that amount. When morphine is present, its effect can be eliminated by using a sample solution containing it as the control without treatment with hydrochloric acid. Accuracy of determination by this method is σ=1.01% (n=6).