1963 年 83 巻 10 号 p. 981-987
Rats were fed on a rice diet containing 0.06% of 4-dimethylaminoazobenzene (DAB) and their urine was examined by two-dimensional paper chromatography. In normal rats, free amino acids in the urine were approximately seven kinds, glycine being the most abundant, and including alanine, glutamic acid, cystine, arginine, histidine, and leucine. In the urine of DAB-administered rats, kind and amount of amino acids increased with increasing dosage of DAB given. The amount of tyrosine and kynu-renine tended to increase markedly.
Quantitative determination was made on the total nitrogen, free tyrosine, and free kynurenine in the 24-hour urine of one rat per week during 16 weeks after the start of administration of DAB, corresponding to the period for formation of liver tumor. There was no great variation in the amount of total nitrogen in the urine during this period but the amount of free tyrosine and free kynurenine was found to increase markedly parallel with the preceding formation of a tumor.