フェノキサゾン化合物の生体物理化学的研究 (第5報)

ウシ眼球内温度と膜透過との関係

抄録

Membrane permeation was tested with bovine eye-lens capsule at 18, 25, and 37°, and the values of permeability constant (P) of the membrane and apparent membrane constant (f*) or (P/D) were calculated. The value of f* showed a maximum at 25° and the rate of decrease of f* showed a minimum value at 25°. It seemed, therefore, that the optimal temperature for permeation through this capsule is between 25° and 37°, and that this temperature probably agrees with the temperature of the anterior capsule in the living body. This temperature was measured with an electric thermistor-type thermometer and it was found to be 33.9° in bovines of 3-4 years of age.
The values of the diffusion constant (D) and degree of association (n) of Phenoxazone compounds, and of P and f* were measured at 30° and 33.9°, and the value of f* showed a maximum at 33.9° in all the dyes tested.
When membrane permeation was carried out using sodium chloride or when permeation of sodium chloride was measured after pretreatment with a dye, the relationship between permeation time and the logarithm of membrane constant was linear. Calculation of the rate of decrease of membrane constant at each temperature from the slope of this straight line showed that the rate was the smallest at 33.9°.
These experimental resultss have shown that membrane permeation of phenoxazone compounds through the bovine eye-lens capsule is the most facile at the biological temperature of the anterior capsule and that the clogging of membrane pores with sodium chloride hardly occurred at this temperature.