アルカロイドのケイ光分析研究 (第1報)

アトロピンのケイ光による新定量法

抄録

A new quantitative method of atropine by fluorophotometric determination has been deviced. A sample which contains 1-100μg. of atropine is evaporated to dryness, and to this 0.5cc. of nitric acid is added. It is also evaporated to dryness on a water-bath, similarly to the treatment in the Vitali reaction. The residue is dissolved in 1cc. of water, basified by 10% sodium hydroxide, and it is reduced by zinc-grains. After the reduction for half an hour, the reduced product is diluted to the definite volume with a ethanol-water mixture (1:1) and the intensity of the fluorescence is measured. Simultaneously the standard and blank are treated similarly followed by the calculation of atropine contents in the sample. As to the filters for the measurement, Matsuda UV-D₂ is used for the excited light sorce, AKA-FL-B₁, for the selection of fluorescence and AKA-UV-O₁, for shattering the excited fluorescence. The light sorces for the excitation is the Toshiba super high-voltage merculy lamp. In this measurement, the fluorescence of atropine is stable, and the intensity shows the Iinearlity in 1-100μg. The presumable standard error for the recurrent line is considered to be σ=0.1048 (=0.3μg. Atropine).