1964 年 84 巻 5 号 p. 390-394
Oxidation of cholesterol in acetone was investigated with the use of the Kiliani reagent at 0° and Cholest-4-ene-3, 6-dione (IV) was prepared in 72% yield with excess reagent.
2, 4-Dinitrophenylhydrazones and p-nitrophenylhydrazones of these ketosteroids (II, III, IV) were prepared, purified, and characterized by the determination of their elemental compositions, melting point, and visible absorptions. Cholest-4-ene-3, 6-dione mono (p-nitrophenylhydrazone) (X) in dimethylformamide shows an absorption maximum at 612mμ in sodium hydroxide solution and has an apparent molecular extinction coefficient of about 64, 400. The present work suggested a possibility to develop a novel method for the determination of cholesterol.