1964 年 84 巻 5 号 p. 435-440
In order to study the precipitation conditions of soluble proteins in bovine lens cortex, precipitation titration with the aid of light scattering apparatus was examined by the use of acetic acid, hydrochloric acid, acetone, and ammonium sulfate as a precipitant to be added continuously to the protein solution in the cell at 5° and 34°. Transmitted light in the direction of 0°, and scattered light in the direction of 45°, 90°, and 135° were recorded. At directions of 0°, 90° and 135°, the number of maximum point in the precipitation titration curve was one. The scattered light at the direction of 45°, on the contrary, had two maximum points, for each precipitant and temperature. It was also found that the first maximum was located at pH 5.5 and the second at pH 4.5. The minimum point between the first and the second was observed at pH 5.0, the value corresponding to the maximum point in the direction of 90° and 135°.