1964 年 84 巻 8 号 p. 783-785
1, 3, 5-Trinitrobenzene was easy to use and sensitive enough to detect cardiac glycosides on paper or thinlayer chromatograms.
Trinitrobenzene solution (0.1% in a mixture of dimethylformamide and water) and sodium carbonate solution (5% in water) are successively sprayed on the developed chromatogram and this is heated at 90-100° for 4-5 minutes. When cooled sodium dihydrogenphosphate solution (5% in water) is sprayed by which cardiac glycoside is revealed as an orange-red spot on an almost colorless background. The limit of detection of some cardiac glycosides is tabulated in Tables I and II.